The test system for the diagnosis of subclinical mastitis of cattle "MASTITEST-S"
The test system for the diagnosis of subclinical mastitis of cattle “MASTITEST-S”
- Type of: Anti-Mastitis Products
Description
Composition. Sulfanol 10%, bromocresol purple, distilled water.
Pharmaceutical form. Residue-free liquid of cherry-violet colour with surface-active agent and indicator. Produced in the form of 10% solution.
Pharmaceutical properties. Testing system action is conditioned by cooperation of sulfanol with somatic cells (white blood cells mostly), contained in the milk during the inflammatory processes in the udder of mastitic cows and the milk pH, indicated due bromocresol purple.
Sulfanol interacts with somatic cells in the milk (white blood cells mostly) generating gelatiniform clot of different density. The more somatic cells on the milk, the denser clot.
Animal species. Cattle (cows), small cattle (sheep, goats).
Therapeutic indications. The drug is intended for disguised inflammations of the udder and treatment results assessment by cows, goats, sheep.
Precautions. Do not use the drug after the expiry date or without necessary flask marking. During the shelf life of the test system by the temperature below +10°С in the flask the precipitation is possible without affecting the test system quality. By residue appearance, the flask is heated by the water bath and stirred up to residue dilution.
Application directions.
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Testing is conducted on the milk-check plate or comparable.
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First, milk yield samples of every cow are tested by 10% solution of Mastitest‑S during the control milk yielding: 1cm3 of milk tested is put into the cavity on the check plate, 1 сm3 of Mastitest‑S 10% solution is added, stirred by the stick during 15-20 sec. The reaction results are defined by the stage of gelatiniform clot generating (by white blood cells increase) and blend colour change (by pH change).
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The milk with positive or doubtful reaction after Mastitest‑S 10% solution used is tested further: the parenchymal milk of every udder quarter is tested by Mastitest S 2% solution.
After milking operation 1 ml of milk of every udder quarter is blended with 1 ml of Mastitest S 2% solution in every check plate cavity; mixed with the stick during 15-20 sec; the reaction is considered similarly to that in the point 2 (Mastitest‑S 10%).
Reaction results assessment
Reaction type |
Reaction intensity marking |
Assessment by the milk consistence |
Assessment by the colour |
Negative reaction |
(-) |
homogeneous fluid |
from light purple to purple |
Disputable reaction |
(+) |
traces of gelatiniform clot generating |
from light purple to deep purple |
(++) |
weak gelatiniform clot generating, dragging after the stick without leaving the fluid |
from light purple to violet |
|
Positive reaction |
(+++) |
moderate gelatiniform clot generating similar to a raw egg albumen, partially leaving the fluid with the stick after mixing |
from deep purple to violet |
(++++) |
shaped gelatiniform clot generating, totally leaving the fluid with the stick |
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Monitoring result records
